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Gary Schroth, PhD provides an introduction to index hopping, describes how to attenuate index hopping degrees by next library prep best practices, and discusses how to remove index-hopped reads utilizing distinctive twin indexing mixtures.
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csv file or perhaps the SampleSheet.csv file established by LRM on the Investigation commence. LRM will detect the instrument type through the operate data files and create a last sample sheet Along with the i5 indexes in the proper orientation for that System.
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When using LRM, i5 sequences needs to be entered into these program within the ahead orientation in all situation and for all instrument varieties.
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When examining index sets for coloration equilibrium, the sequence noticed via the instrument must be assessed, regardless of the orientation used for the sample sheet. For your i7 index, usually asses color harmony utilizing the "i7 bases for sample sheet" column orientation.
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